Reactivity
Clone FX3 has been developed and validated for fluorescence multiplex IHC studies of FOXP3 expression in human tissues.
FOXP3 (Forkhead box protein P3) is mainly expressed in Regulatory T (Treg) cells, a subset of CD4+ T-cells, that play a suppressive role in the immune system. Treg cells ensure immune homeostasis through their ability to suppress the activation and function of leukocytes. FOXP3 has emerged as a prominent target for the development of new immunotherapies for cancer and autoimmune diseases.
			 
					
				IHC protocols
Staining protocols for anti-human FOXP3 antibody clone FX3
| Cat.No.: | 
DIA-FX3 | 
| Isotype: | 
Mouse IgG2a/k | 
| Specificity: | 
Human FOXP3 | 
| Physical State: | 
Lyophilized powder | 
| Reconstitution: | 
DIA-FX3, restore to 100µl. Reconstitute with sterile destilled water by gentle shaking for 10 minutes. | 
 
Manual stain with autoclave
- Pretreatment buffer: 121°C / 5 min / pH7,8
 
- Incubation primary antibody: 60 min / 37°C (Dilution: 1:100)
 
- Envision HRP rabbit/mouse: 30 min / 37°C
 
 
Applicaple for automated staining procedures and validated for mulitcolor immunofluorescebnce (multiplexed IHC).
			 
					
				References
Scientific publications for anti-FOXP3 clone FX3 have been submitted and reference data will be available soon.