FOXP3

492,00 plus VAT

#DIA-FX3
Anti-FOXP3 (HU) from Mouse (clone FX3) – 100 µl
Category:

Description

Cat.No.DIA-FX3
SpecificityFOXP3
Species ReactivityHuman
Host SpeciesMouse
IsotypeIgG2a/k
CloneFX3
Application
Immunohistochemistry (IHC),
Formalin-fixed Paraffin-embedded sections (FFPE)
Western Blot
Conjugationunconjugated
DilutionIHC 1:100-1:200
Format
Lyophilisate,
Antibody purified (from culture supernatant)
Quantity100µl
Positive controlTonsil
PresentationPBS pH 7.4, 2%BSA, 0.05%NaN3
Intended UseResearch Use Only
Manufacturer / BrandONCOdianova
FOXP3 IHC-Gallery
IHC-figures on FOXP3 detection in different tumors.

Reactivity

Clone FX3 has been developed and validated for fluorescence multiplex IHC studies of FOXP3 expression in human tissues.

FOXP3 (Forkhead box protein P3) is mainly expressed in Regulatory T (Treg) cells, a subset of CD4+ T-cells, that play a suppressive role in the immune system. Treg cells ensure immune homeostasis through their ability to suppress the activation and function of leukocytes. FOXP3 has emerged as a prominent target for the development of new immunotherapies for cancer and autoimmune diseases.

IHC protocols

Staining protocols for anti-human FOXP3 antibody clone FX3
Cat.No.:DIA-FX3
Isotype:Mouse IgG2a/k
Specificity:Human FOXP3
Physical State:Lyophilized powder
Reconstitution:DIA-FX3, restore to 100µl. Reconstitute with sterile destilled water by gentle shaking for 10 minutes.
Manual stain with autoclave
  • Pretreatment buffer: 121°C / 5 min / pH7,8
  • Incubation primary antibody: 60 min / 37°C (Dilution: 1:100)
  • Envision HRP rabbit/mouse: 30 min / 37°C
Applicaple for automated staining procedures and validated for mulitcolor immunofluorescebnce (multiplexed IHC).

References

Scientific publications for anti-FOXP3 clone FX3 have been submitted and reference data will be available soon.

FOXP3 IHC-Gallery
IHC-figures on FOXP3 detection in different tumors.

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