FOXP3

492,00 plus VAT

#DIA-FX3
Anti-FOXP3 (HU) aus Maus (Klon FX3) – 100 µl

Beschreibung

Prod.Nr. DIA-FX3
Spezifität FOXP3
Speziesreaktivität Human
Wirtsspezies Mouse
Isotyp IgG2a/k
Klon FX3
Anwendung
Immunohistochemistry (IHC),
Formalin-fixed Paraffin-embedded sections (FFPE)
Western Blot
Konjugation unconjugated
Verdünnung IHC 1:100-1:200
Format
Lyophilisate,
Antibody purified (from culture supernatant)
Anzahl 100µl
Positive control Tonsil
Format PBS pH 7.4, 2%BSA, 0.05%NaN3
Anwendungshinweis Research Use Only
Hersteller ONCOdianova
FOXP3 IHC-Gallery
IHC-figures on FOXP3 detection in different tumors.

Reaktivität

Clone FX3 has been developed and validated for fluorescence multiplex IHC studies of FOXP3 expression in human tissues.

FOXP3 (Forkhead box protein P3) is mainly expressed in Regulatory T (Treg) cells, a subset of CD4+ T-cells, that play a suppressive role in the immune system. Treg cells ensure immune homeostasis through their ability to suppress the activation and function of leukocytes. FOXP3 has emerged as a prominent target for the development of new immunotherapies for cancer and autoimmune diseases.

IHC Protokoll

Staining protocols for anti-human FOXP3 antibody clone FX3
Cat.No.: DIA-FX3
Isotype: Mouse IgG2a/k
Specificity: Human FOXP3
Physical State: Lyophilized powder
Reconstitution: DIA-FX3, restore to 100µl. Reconstitute with sterile destilled water by gentle shaking for 10 minutes.
Manual stain with autoclave
  • Pretreatment buffer: 121°C / 5 min / pH7,8
  • Incubation primary antibody: 60 min / 37°C (Dilution: 1:100)
  • Envision HRP rabbit/mouse: 30 min / 37°C
Applicaple for automated staining procedures and validated for mulitcolor immunofluorescebnce (multiplexed IHC).

Referenzen

Scientific publications for anti-FOXP3 clone FX3 have been submitted and reference data will be available soon.

FOXP3 IHC-Gallery
IHC-figures on FOXP3 detection in different tumors.
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